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    Structured Review

    Nikon confocal laser scanning microscopy
    Confocal Laser Scanning Microscopy, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/confocal+laser-scanning+microscopy/C2%2B/pmc12914669-48-15-19
    Average 99 stars, based on 1 article reviews
    confocal laser scanning microscopy - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Black tantalic oxide submicro-particles coating on PEEK fibers woven into fabrics as artificial ligaments with photothermal antibacterial effect and osteogenic activity for promoting ligament-bone healing
    Article Snippet: Design of artificial ligaments possessing both osteogenic activity and antibacterial effect that promotes ligament-bone healing and prevents bacterial infection in bone tunnels for anterior cruciate ligament (ACL) reconstruction remains a significant challenge.. In this study, black tantalic oxide (BTO) submicroparticles with oxygen vacancies and structure defects were fabricated by using traditional white tantalic oxide (WTO) through magnesium thermal reduction (MTR) method, and BTO was coated on polyetheretherketone (PEEK) fibers (PKF), which were woven into fabrics (PBT) as artificial ligaments.. PBT with BTO coating exhibited excellent photothermal performance, which possessed not only antibacterial effects in vitro but also anti-infective ability in vivo .

    Article Title: Engineering sialylated N-glycans on adeno-associated virus capsids for targeted gene delivery and therapeutic applications.
    Article Snippet: Glycans with diverse biological functions have been extensively identified on enveloped viruses, whereas glycosylation on adeno-associated virus (AAV) serotypes remains poorly understood.. Identifying potential glycosylation sites on AAVs could provide critical docking sites for rational engineering of AAV capsids, enabling targeted delivery of therapeutic genes.. This study presents a strategy that integrates azido-monosaccharide metabolic incorporation, 1,2-diamino-4,5-methylenedioxybenzene-labeled sialic acid analysis, and mass spectrometry to identify N-glycosylation sites and glycoforms on AAVs.

    Article Title: Nanocrystalline Cellulose Modulates Dysregulated Intestinal Barriers in Ulcerative Colitis.
    Article Snippet: Ulcerative colitis (UC) is a recurrent chronic inflammation of the colon with increasing incidence and prevalence, which could increase the risk of colorectal cancer.. It is urgent to find an effective method with few side effects.. Nanocrystalline cellulose (NCC), which is from plant fibers, has a good biocompatibility and high biosafety.

    Article Title: MiR-206 may regulate mitochondrial ROS contribute to the progression of Myocardial infarction via TREM1.
    Article Snippet: And then images of fluorescent were observed and collected by Confocal laser-scanning microscopy (Nikon).

    Article Title: XA pH-Responsive and Colitis-Targeted Nanoparticle Loaded with Shikonin for the Oral Treatment of Inflammatory Bowel Disease in Mice.
    Article Snippet: Epidemiology shows that more than 6.8 million people in the world are influenced by inflammatory bowel disease (IBD) each year.. IBD is a refractory inflammatory disease, and the disease mainly affects the colon.. Shikonin (SK) was originally extracted from traditional Chinese medicine “Zicao” (with an English name Lithospermum erythrorhizon) and found to inhibit inflammation, regulate immunity, and be involved in healing wounds.

    Article Title: Hyaluronic acid-bilirubin nanomedicine-based combination chemoimmunotherapy.
    Article Snippet: For CRT or PD-L1 marker study, MC38 or 4T1 cells in culture medium were treated with SC144 (10μM),HABN (40μg/ml) or SC144@HABN (10μMof SC144; 40μg/ml of HABN) for 20 h. For preparation of confocal samples before confocal microscopy analysis, 20 μg/ml of HABN-Cy5.5, FITC conjugated anti-CD44 antibody (1:100 dilution), PE-conjugated calreticulin antibody (1:100 dilution), PE-conjugated anti-PD-L1 antibody (1:100 dilution) or control medium was treated with the cells for 1 h. The cells were then fixed with 4% PFA for 5min, counter-stained with Hoechst 33342 for 10min and analyzed by confocal laser-scanning microscopy (Nikon A1).


    Article Title: Arabidopsis OTU2 deubiquitinates cysteine protease RD21A to enhance clubroot resistance
    Article Snippet: A. tumefaciens GV3101 strains carrying the target plasmid are transiently expressed in the N. benthamiana and observed 48 h after A. tumefaciens GV3101 infiltration, detected with a confocal laser‐scanning microscopy (Nikon; A1HD25).

    Confocal Laser Scanning Microscopy:

    Article Title: Black tantalic oxide submicro-particles coating on PEEK fibers woven into fabrics as artificial ligaments with photothermal antibacterial effect and osteogenic activity for promoting ligament-bone healing
    Article Snippet: Design of artificial ligaments possessing both osteogenic activity and antibacterial effect that promotes ligament-bone healing and prevents bacterial infection in bone tunnels for anterior cruciate ligament (ACL) reconstruction remains a significant challenge.. In this study, black tantalic oxide (BTO) submicroparticles with oxygen vacancies and structure defects were fabricated by using traditional white tantalic oxide (WTO) through magnesium thermal reduction (MTR) method, and BTO was coated on polyetheretherketone (PEEK) fibers (PKF), which were woven into fabrics (PBT) as artificial ligaments.. PBT with BTO coating exhibited excellent photothermal performance, which possessed not only antibacterial effects in vitro but also anti-infective ability in vivo .

    Article Title: Engineering sialylated N-glycans on adeno-associated virus capsids for targeted gene delivery and therapeutic applications.
    Article Snippet: Glycans with diverse biological functions have been extensively identified on enveloped viruses, whereas glycosylation on adeno-associated virus (AAV) serotypes remains poorly understood.. Identifying potential glycosylation sites on AAVs could provide critical docking sites for rational engineering of AAV capsids, enabling targeted delivery of therapeutic genes.. This study presents a strategy that integrates azido-monosaccharide metabolic incorporation, 1,2-diamino-4,5-methylenedioxybenzene-labeled sialic acid analysis, and mass spectrometry to identify N-glycosylation sites and glycoforms on AAVs.

    Article Title: Nanocrystalline Cellulose Modulates Dysregulated Intestinal Barriers in Ulcerative Colitis.
    Article Snippet: Ulcerative colitis (UC) is a recurrent chronic inflammation of the colon with increasing incidence and prevalence, which could increase the risk of colorectal cancer.. It is urgent to find an effective method with few side effects.. Nanocrystalline cellulose (NCC), which is from plant fibers, has a good biocompatibility and high biosafety.

    Article Title: MiR-206 may regulate mitochondrial ROS contribute to the progression of Myocardial infarction via TREM1.
    Article Snippet: And then images of fluorescent were observed and collected by Confocal laser-scanning microscopy (Nikon).

    Article Title: XA pH-Responsive and Colitis-Targeted Nanoparticle Loaded with Shikonin for the Oral Treatment of Inflammatory Bowel Disease in Mice.
    Article Snippet: Epidemiology shows that more than 6.8 million people in the world are influenced by inflammatory bowel disease (IBD) each year.. IBD is a refractory inflammatory disease, and the disease mainly affects the colon.. Shikonin (SK) was originally extracted from traditional Chinese medicine “Zicao” (with an English name Lithospermum erythrorhizon) and found to inhibit inflammation, regulate immunity, and be involved in healing wounds.

    Article Title: Hyaluronic acid-bilirubin nanomedicine-based combination chemoimmunotherapy.
    Article Snippet: For CRT or PD-L1 marker study, MC38 or 4T1 cells in culture medium were treated with SC144 (10μM),HABN (40μg/ml) or SC144@HABN (10μMof SC144; 40μg/ml of HABN) for 20 h. For preparation of confocal samples before confocal microscopy analysis, 20 μg/ml of HABN-Cy5.5, FITC conjugated anti-CD44 antibody (1:100 dilution), PE-conjugated calreticulin antibody (1:100 dilution), PE-conjugated anti-PD-L1 antibody (1:100 dilution) or control medium was treated with the cells for 1 h. The cells were then fixed with 4% PFA for 5min, counter-stained with Hoechst 33342 for 10min and analyzed by confocal laser-scanning microscopy (Nikon A1).


    Article Title: Arabidopsis OTU2 deubiquitinates cysteine protease RD21A to enhance clubroot resistance
    Article Snippet: A. tumefaciens GV3101 strains carrying the target plasmid are transiently expressed in the N. benthamiana and observed 48 h after A. tumefaciens GV3101 infiltration, detected with a confocal laser‐scanning microscopy (Nikon; A1HD25).

    Expressing:

    Article Title: Black tantalic oxide submicro-particles coating on PEEK fibers woven into fabrics as artificial ligaments with photothermal antibacterial effect and osteogenic activity for promoting ligament-bone healing
    Article Snippet: Design of artificial ligaments possessing both osteogenic activity and antibacterial effect that promotes ligament-bone healing and prevents bacterial infection in bone tunnels for anterior cruciate ligament (ACL) reconstruction remains a significant challenge.. In this study, black tantalic oxide (BTO) submicroparticles with oxygen vacancies and structure defects were fabricated by using traditional white tantalic oxide (WTO) through magnesium thermal reduction (MTR) method, and BTO was coated on polyetheretherketone (PEEK) fibers (PKF), which were woven into fabrics (PBT) as artificial ligaments.. PBT with BTO coating exhibited excellent photothermal performance, which possessed not only antibacterial effects in vitro but also anti-infective ability in vivo .

    Article Title: Engineering sialylated N-glycans on adeno-associated virus capsids for targeted gene delivery and therapeutic applications.
    Article Snippet: Glycans with diverse biological functions have been extensively identified on enveloped viruses, whereas glycosylation on adeno-associated virus (AAV) serotypes remains poorly understood.. Identifying potential glycosylation sites on AAVs could provide critical docking sites for rational engineering of AAV capsids, enabling targeted delivery of therapeutic genes.. This study presents a strategy that integrates azido-monosaccharide metabolic incorporation, 1,2-diamino-4,5-methylenedioxybenzene-labeled sialic acid analysis, and mass spectrometry to identify N-glycosylation sites and glycoforms on AAVs.

    Article Title: Nanocrystalline Cellulose Modulates Dysregulated Intestinal Barriers in Ulcerative Colitis.
    Article Snippet: Ulcerative colitis (UC) is a recurrent chronic inflammation of the colon with increasing incidence and prevalence, which could increase the risk of colorectal cancer.. It is urgent to find an effective method with few side effects.. Nanocrystalline cellulose (NCC), which is from plant fibers, has a good biocompatibility and high biosafety.

    Article Title: MiR-206 may regulate mitochondrial ROS contribute to the progression of Myocardial infarction via TREM1.
    Article Snippet: And then images of fluorescent were observed and collected by Confocal laser-scanning microscopy (Nikon).

    Article Title: XA pH-Responsive and Colitis-Targeted Nanoparticle Loaded with Shikonin for the Oral Treatment of Inflammatory Bowel Disease in Mice.
    Article Snippet: Epidemiology shows that more than 6.8 million people in the world are influenced by inflammatory bowel disease (IBD) each year.. IBD is a refractory inflammatory disease, and the disease mainly affects the colon.. Shikonin (SK) was originally extracted from traditional Chinese medicine “Zicao” (with an English name Lithospermum erythrorhizon) and found to inhibit inflammation, regulate immunity, and be involved in healing wounds.

    Article Title: Hyaluronic acid-bilirubin nanomedicine-based combination chemoimmunotherapy.
    Article Snippet: For CRT or PD-L1 marker study, MC38 or 4T1 cells in culture medium were treated with SC144 (10μM),HABN (40μg/ml) or SC144@HABN (10μMof SC144; 40μg/ml of HABN) for 20 h. For preparation of confocal samples before confocal microscopy analysis, 20 μg/ml of HABN-Cy5.5, FITC conjugated anti-CD44 antibody (1:100 dilution), PE-conjugated calreticulin antibody (1:100 dilution), PE-conjugated anti-PD-L1 antibody (1:100 dilution) or control medium was treated with the cells for 1 h. The cells were then fixed with 4% PFA for 5min, counter-stained with Hoechst 33342 for 10min and analyzed by confocal laser-scanning microscopy (Nikon A1).


    Article Title: Arabidopsis OTU2 deubiquitinates cysteine protease RD21A to enhance clubroot resistance
    Article Snippet: A. tumefaciens GV3101 strains carrying the target plasmid are transiently expressed in the N. benthamiana and observed 48 h after A. tumefaciens GV3101 infiltration, detected with a confocal laser‐scanning microscopy (Nikon; A1HD25).

    Software:

    Article Title: Black tantalic oxide submicro-particles coating on PEEK fibers woven into fabrics as artificial ligaments with photothermal antibacterial effect and osteogenic activity for promoting ligament-bone healing
    Article Snippet: Design of artificial ligaments possessing both osteogenic activity and antibacterial effect that promotes ligament-bone healing and prevents bacterial infection in bone tunnels for anterior cruciate ligament (ACL) reconstruction remains a significant challenge.. In this study, black tantalic oxide (BTO) submicroparticles with oxygen vacancies and structure defects were fabricated by using traditional white tantalic oxide (WTO) through magnesium thermal reduction (MTR) method, and BTO was coated on polyetheretherketone (PEEK) fibers (PKF), which were woven into fabrics (PBT) as artificial ligaments.. PBT with BTO coating exhibited excellent photothermal performance, which possessed not only antibacterial effects in vitro but also anti-infective ability in vivo .

    Article Title: Engineering sialylated N-glycans on adeno-associated virus capsids for targeted gene delivery and therapeutic applications.
    Article Snippet: Glycans with diverse biological functions have been extensively identified on enveloped viruses, whereas glycosylation on adeno-associated virus (AAV) serotypes remains poorly understood.. Identifying potential glycosylation sites on AAVs could provide critical docking sites for rational engineering of AAV capsids, enabling targeted delivery of therapeutic genes.. This study presents a strategy that integrates azido-monosaccharide metabolic incorporation, 1,2-diamino-4,5-methylenedioxybenzene-labeled sialic acid analysis, and mass spectrometry to identify N-glycosylation sites and glycoforms on AAVs.

    Article Title: Nanocrystalline Cellulose Modulates Dysregulated Intestinal Barriers in Ulcerative Colitis.
    Article Snippet: Ulcerative colitis (UC) is a recurrent chronic inflammation of the colon with increasing incidence and prevalence, which could increase the risk of colorectal cancer.. It is urgent to find an effective method with few side effects.. Nanocrystalline cellulose (NCC), which is from plant fibers, has a good biocompatibility and high biosafety.

    Article Title: MiR-206 may regulate mitochondrial ROS contribute to the progression of Myocardial infarction via TREM1.
    Article Snippet: And then images of fluorescent were observed and collected by Confocal laser-scanning microscopy (Nikon).

    Article Title: XA pH-Responsive and Colitis-Targeted Nanoparticle Loaded with Shikonin for the Oral Treatment of Inflammatory Bowel Disease in Mice.
    Article Snippet: Epidemiology shows that more than 6.8 million people in the world are influenced by inflammatory bowel disease (IBD) each year.. IBD is a refractory inflammatory disease, and the disease mainly affects the colon.. Shikonin (SK) was originally extracted from traditional Chinese medicine “Zicao” (with an English name Lithospermum erythrorhizon) and found to inhibit inflammation, regulate immunity, and be involved in healing wounds.

    Article Title: Hyaluronic acid-bilirubin nanomedicine-based combination chemoimmunotherapy.
    Article Snippet: For CRT or PD-L1 marker study, MC38 or 4T1 cells in culture medium were treated with SC144 (10μM),HABN (40μg/ml) or SC144@HABN (10μMof SC144; 40μg/ml of HABN) for 20 h. For preparation of confocal samples before confocal microscopy analysis, 20 μg/ml of HABN-Cy5.5, FITC conjugated anti-CD44 antibody (1:100 dilution), PE-conjugated calreticulin antibody (1:100 dilution), PE-conjugated anti-PD-L1 antibody (1:100 dilution) or control medium was treated with the cells for 1 h. The cells were then fixed with 4% PFA for 5min, counter-stained with Hoechst 33342 for 10min and analyzed by confocal laser-scanning microscopy (Nikon A1).


    Article Title: Arabidopsis OTU2 deubiquitinates cysteine protease RD21A to enhance clubroot resistance
    Article Snippet: A. tumefaciens GV3101 strains carrying the target plasmid are transiently expressed in the N. benthamiana and observed 48 h after A. tumefaciens GV3101 infiltration, detected with a confocal laser‐scanning microscopy (Nikon; A1HD25).

    Quantitative RT-PCR:

    Article Title: Black tantalic oxide submicro-particles coating on PEEK fibers woven into fabrics as artificial ligaments with photothermal antibacterial effect and osteogenic activity for promoting ligament-bone healing
    Article Snippet: Design of artificial ligaments possessing both osteogenic activity and antibacterial effect that promotes ligament-bone healing and prevents bacterial infection in bone tunnels for anterior cruciate ligament (ACL) reconstruction remains a significant challenge.. In this study, black tantalic oxide (BTO) submicroparticles with oxygen vacancies and structure defects were fabricated by using traditional white tantalic oxide (WTO) through magnesium thermal reduction (MTR) method, and BTO was coated on polyetheretherketone (PEEK) fibers (PKF), which were woven into fabrics (PBT) as artificial ligaments.. PBT with BTO coating exhibited excellent photothermal performance, which possessed not only antibacterial effects in vitro but also anti-infective ability in vivo .

    Article Title: Engineering sialylated N-glycans on adeno-associated virus capsids for targeted gene delivery and therapeutic applications.
    Article Snippet: Glycans with diverse biological functions have been extensively identified on enveloped viruses, whereas glycosylation on adeno-associated virus (AAV) serotypes remains poorly understood.. Identifying potential glycosylation sites on AAVs could provide critical docking sites for rational engineering of AAV capsids, enabling targeted delivery of therapeutic genes.. This study presents a strategy that integrates azido-monosaccharide metabolic incorporation, 1,2-diamino-4,5-methylenedioxybenzene-labeled sialic acid analysis, and mass spectrometry to identify N-glycosylation sites and glycoforms on AAVs.

    Article Title: Nanocrystalline Cellulose Modulates Dysregulated Intestinal Barriers in Ulcerative Colitis.
    Article Snippet: Ulcerative colitis (UC) is a recurrent chronic inflammation of the colon with increasing incidence and prevalence, which could increase the risk of colorectal cancer.. It is urgent to find an effective method with few side effects.. Nanocrystalline cellulose (NCC), which is from plant fibers, has a good biocompatibility and high biosafety.

    Article Title: MiR-206 may regulate mitochondrial ROS contribute to the progression of Myocardial infarction via TREM1.
    Article Snippet: And then images of fluorescent were observed and collected by Confocal laser-scanning microscopy (Nikon).

    Article Title: XA pH-Responsive and Colitis-Targeted Nanoparticle Loaded with Shikonin for the Oral Treatment of Inflammatory Bowel Disease in Mice.
    Article Snippet: Epidemiology shows that more than 6.8 million people in the world are influenced by inflammatory bowel disease (IBD) each year.. IBD is a refractory inflammatory disease, and the disease mainly affects the colon.. Shikonin (SK) was originally extracted from traditional Chinese medicine “Zicao” (with an English name Lithospermum erythrorhizon) and found to inhibit inflammation, regulate immunity, and be involved in healing wounds.

    Article Title: Hyaluronic acid-bilirubin nanomedicine-based combination chemoimmunotherapy.
    Article Snippet: For CRT or PD-L1 marker study, MC38 or 4T1 cells in culture medium were treated with SC144 (10μM),HABN (40μg/ml) or SC144@HABN (10μMof SC144; 40μg/ml of HABN) for 20 h. For preparation of confocal samples before confocal microscopy analysis, 20 μg/ml of HABN-Cy5.5, FITC conjugated anti-CD44 antibody (1:100 dilution), PE-conjugated calreticulin antibody (1:100 dilution), PE-conjugated anti-PD-L1 antibody (1:100 dilution) or control medium was treated with the cells for 1 h. The cells were then fixed with 4% PFA for 5min, counter-stained with Hoechst 33342 for 10min and analyzed by confocal laser-scanning microscopy (Nikon A1).


    Article Title: Arabidopsis OTU2 deubiquitinates cysteine protease RD21A to enhance clubroot resistance
    Article Snippet: A. tumefaciens GV3101 strains carrying the target plasmid are transiently expressed in the N. benthamiana and observed 48 h after A. tumefaciens GV3101 infiltration, detected with a confocal laser‐scanning microscopy (Nikon; A1HD25).

    Marker:

    Article Title: Black tantalic oxide submicro-particles coating on PEEK fibers woven into fabrics as artificial ligaments with photothermal antibacterial effect and osteogenic activity for promoting ligament-bone healing
    Article Snippet: Design of artificial ligaments possessing both osteogenic activity and antibacterial effect that promotes ligament-bone healing and prevents bacterial infection in bone tunnels for anterior cruciate ligament (ACL) reconstruction remains a significant challenge.. In this study, black tantalic oxide (BTO) submicroparticles with oxygen vacancies and structure defects were fabricated by using traditional white tantalic oxide (WTO) through magnesium thermal reduction (MTR) method, and BTO was coated on polyetheretherketone (PEEK) fibers (PKF), which were woven into fabrics (PBT) as artificial ligaments.. PBT with BTO coating exhibited excellent photothermal performance, which possessed not only antibacterial effects in vitro but also anti-infective ability in vivo .

    Article Title: Engineering sialylated N-glycans on adeno-associated virus capsids for targeted gene delivery and therapeutic applications.
    Article Snippet: Glycans with diverse biological functions have been extensively identified on enveloped viruses, whereas glycosylation on adeno-associated virus (AAV) serotypes remains poorly understood.. Identifying potential glycosylation sites on AAVs could provide critical docking sites for rational engineering of AAV capsids, enabling targeted delivery of therapeutic genes.. This study presents a strategy that integrates azido-monosaccharide metabolic incorporation, 1,2-diamino-4,5-methylenedioxybenzene-labeled sialic acid analysis, and mass spectrometry to identify N-glycosylation sites and glycoforms on AAVs.

    Article Title: Nanocrystalline Cellulose Modulates Dysregulated Intestinal Barriers in Ulcerative Colitis.
    Article Snippet: Ulcerative colitis (UC) is a recurrent chronic inflammation of the colon with increasing incidence and prevalence, which could increase the risk of colorectal cancer.. It is urgent to find an effective method with few side effects.. Nanocrystalline cellulose (NCC), which is from plant fibers, has a good biocompatibility and high biosafety.

    Article Title: MiR-206 may regulate mitochondrial ROS contribute to the progression of Myocardial infarction via TREM1.
    Article Snippet: And then images of fluorescent were observed and collected by Confocal laser-scanning microscopy (Nikon).

    Article Title: XA pH-Responsive and Colitis-Targeted Nanoparticle Loaded with Shikonin for the Oral Treatment of Inflammatory Bowel Disease in Mice.
    Article Snippet: Epidemiology shows that more than 6.8 million people in the world are influenced by inflammatory bowel disease (IBD) each year.. IBD is a refractory inflammatory disease, and the disease mainly affects the colon.. Shikonin (SK) was originally extracted from traditional Chinese medicine “Zicao” (with an English name Lithospermum erythrorhizon) and found to inhibit inflammation, regulate immunity, and be involved in healing wounds.

    Article Title: Hyaluronic acid-bilirubin nanomedicine-based combination chemoimmunotherapy.
    Article Snippet: For CRT or PD-L1 marker study, MC38 or 4T1 cells in culture medium were treated with SC144 (10μM),HABN (40μg/ml) or SC144@HABN (10μMof SC144; 40μg/ml of HABN) for 20 h. For preparation of confocal samples before confocal microscopy analysis, 20 μg/ml of HABN-Cy5.5, FITC conjugated anti-CD44 antibody (1:100 dilution), PE-conjugated calreticulin antibody (1:100 dilution), PE-conjugated anti-PD-L1 antibody (1:100 dilution) or control medium was treated with the cells for 1 h. The cells were then fixed with 4% PFA for 5min, counter-stained with Hoechst 33342 for 10min and analyzed by confocal laser-scanning microscopy (Nikon A1).


    Article Title: Arabidopsis OTU2 deubiquitinates cysteine protease RD21A to enhance clubroot resistance
    Article Snippet: A. tumefaciens GV3101 strains carrying the target plasmid are transiently expressed in the N. benthamiana and observed 48 h after A. tumefaciens GV3101 infiltration, detected with a confocal laser‐scanning microscopy (Nikon; A1HD25).

    Confocal Microscopy:

    Article Title: Black tantalic oxide submicro-particles coating on PEEK fibers woven into fabrics as artificial ligaments with photothermal antibacterial effect and osteogenic activity for promoting ligament-bone healing
    Article Snippet: Design of artificial ligaments possessing both osteogenic activity and antibacterial effect that promotes ligament-bone healing and prevents bacterial infection in bone tunnels for anterior cruciate ligament (ACL) reconstruction remains a significant challenge.. In this study, black tantalic oxide (BTO) submicroparticles with oxygen vacancies and structure defects were fabricated by using traditional white tantalic oxide (WTO) through magnesium thermal reduction (MTR) method, and BTO was coated on polyetheretherketone (PEEK) fibers (PKF), which were woven into fabrics (PBT) as artificial ligaments.. PBT with BTO coating exhibited excellent photothermal performance, which possessed not only antibacterial effects in vitro but also anti-infective ability in vivo .

    Article Title: Engineering sialylated N-glycans on adeno-associated virus capsids for targeted gene delivery and therapeutic applications.
    Article Snippet: Glycans with diverse biological functions have been extensively identified on enveloped viruses, whereas glycosylation on adeno-associated virus (AAV) serotypes remains poorly understood.. Identifying potential glycosylation sites on AAVs could provide critical docking sites for rational engineering of AAV capsids, enabling targeted delivery of therapeutic genes.. This study presents a strategy that integrates azido-monosaccharide metabolic incorporation, 1,2-diamino-4,5-methylenedioxybenzene-labeled sialic acid analysis, and mass spectrometry to identify N-glycosylation sites and glycoforms on AAVs.

    Article Title: Nanocrystalline Cellulose Modulates Dysregulated Intestinal Barriers in Ulcerative Colitis.
    Article Snippet: Ulcerative colitis (UC) is a recurrent chronic inflammation of the colon with increasing incidence and prevalence, which could increase the risk of colorectal cancer.. It is urgent to find an effective method with few side effects.. Nanocrystalline cellulose (NCC), which is from plant fibers, has a good biocompatibility and high biosafety.

    Article Title: MiR-206 may regulate mitochondrial ROS contribute to the progression of Myocardial infarction via TREM1.
    Article Snippet: And then images of fluorescent were observed and collected by Confocal laser-scanning microscopy (Nikon).

    Article Title: XA pH-Responsive and Colitis-Targeted Nanoparticle Loaded with Shikonin for the Oral Treatment of Inflammatory Bowel Disease in Mice.
    Article Snippet: Epidemiology shows that more than 6.8 million people in the world are influenced by inflammatory bowel disease (IBD) each year.. IBD is a refractory inflammatory disease, and the disease mainly affects the colon.. Shikonin (SK) was originally extracted from traditional Chinese medicine “Zicao” (with an English name Lithospermum erythrorhizon) and found to inhibit inflammation, regulate immunity, and be involved in healing wounds.

    Article Title: Hyaluronic acid-bilirubin nanomedicine-based combination chemoimmunotherapy.
    Article Snippet: For CRT or PD-L1 marker study, MC38 or 4T1 cells in culture medium were treated with SC144 (10μM),HABN (40μg/ml) or SC144@HABN (10μMof SC144; 40μg/ml of HABN) for 20 h. For preparation of confocal samples before confocal microscopy analysis, 20 μg/ml of HABN-Cy5.5, FITC conjugated anti-CD44 antibody (1:100 dilution), PE-conjugated calreticulin antibody (1:100 dilution), PE-conjugated anti-PD-L1 antibody (1:100 dilution) or control medium was treated with the cells for 1 h. The cells were then fixed with 4% PFA for 5min, counter-stained with Hoechst 33342 for 10min and analyzed by confocal laser-scanning microscopy (Nikon A1).


    Article Title: Arabidopsis OTU2 deubiquitinates cysteine protease RD21A to enhance clubroot resistance
    Article Snippet: A. tumefaciens GV3101 strains carrying the target plasmid are transiently expressed in the N. benthamiana and observed 48 h after A. tumefaciens GV3101 infiltration, detected with a confocal laser‐scanning microscopy (Nikon; A1HD25).

    Control:

    Article Title: Black tantalic oxide submicro-particles coating on PEEK fibers woven into fabrics as artificial ligaments with photothermal antibacterial effect and osteogenic activity for promoting ligament-bone healing
    Article Snippet: Design of artificial ligaments possessing both osteogenic activity and antibacterial effect that promotes ligament-bone healing and prevents bacterial infection in bone tunnels for anterior cruciate ligament (ACL) reconstruction remains a significant challenge.. In this study, black tantalic oxide (BTO) submicroparticles with oxygen vacancies and structure defects were fabricated by using traditional white tantalic oxide (WTO) through magnesium thermal reduction (MTR) method, and BTO was coated on polyetheretherketone (PEEK) fibers (PKF), which were woven into fabrics (PBT) as artificial ligaments.. PBT with BTO coating exhibited excellent photothermal performance, which possessed not only antibacterial effects in vitro but also anti-infective ability in vivo .

    Article Title: Engineering sialylated N-glycans on adeno-associated virus capsids for targeted gene delivery and therapeutic applications.
    Article Snippet: Glycans with diverse biological functions have been extensively identified on enveloped viruses, whereas glycosylation on adeno-associated virus (AAV) serotypes remains poorly understood.. Identifying potential glycosylation sites on AAVs could provide critical docking sites for rational engineering of AAV capsids, enabling targeted delivery of therapeutic genes.. This study presents a strategy that integrates azido-monosaccharide metabolic incorporation, 1,2-diamino-4,5-methylenedioxybenzene-labeled sialic acid analysis, and mass spectrometry to identify N-glycosylation sites and glycoforms on AAVs.

    Article Title: Nanocrystalline Cellulose Modulates Dysregulated Intestinal Barriers in Ulcerative Colitis.
    Article Snippet: Ulcerative colitis (UC) is a recurrent chronic inflammation of the colon with increasing incidence and prevalence, which could increase the risk of colorectal cancer.. It is urgent to find an effective method with few side effects.. Nanocrystalline cellulose (NCC), which is from plant fibers, has a good biocompatibility and high biosafety.

    Article Title: MiR-206 may regulate mitochondrial ROS contribute to the progression of Myocardial infarction via TREM1.
    Article Snippet: And then images of fluorescent were observed and collected by Confocal laser-scanning microscopy (Nikon).

    Article Title: XA pH-Responsive and Colitis-Targeted Nanoparticle Loaded with Shikonin for the Oral Treatment of Inflammatory Bowel Disease in Mice.
    Article Snippet: Epidemiology shows that more than 6.8 million people in the world are influenced by inflammatory bowel disease (IBD) each year.. IBD is a refractory inflammatory disease, and the disease mainly affects the colon.. Shikonin (SK) was originally extracted from traditional Chinese medicine “Zicao” (with an English name Lithospermum erythrorhizon) and found to inhibit inflammation, regulate immunity, and be involved in healing wounds.

    Article Title: Hyaluronic acid-bilirubin nanomedicine-based combination chemoimmunotherapy.
    Article Snippet: For CRT or PD-L1 marker study, MC38 or 4T1 cells in culture medium were treated with SC144 (10μM),HABN (40μg/ml) or SC144@HABN (10μMof SC144; 40μg/ml of HABN) for 20 h. For preparation of confocal samples before confocal microscopy analysis, 20 μg/ml of HABN-Cy5.5, FITC conjugated anti-CD44 antibody (1:100 dilution), PE-conjugated calreticulin antibody (1:100 dilution), PE-conjugated anti-PD-L1 antibody (1:100 dilution) or control medium was treated with the cells for 1 h. The cells were then fixed with 4% PFA for 5min, counter-stained with Hoechst 33342 for 10min and analyzed by confocal laser-scanning microscopy (Nikon A1).


    Article Title: Arabidopsis OTU2 deubiquitinates cysteine protease RD21A to enhance clubroot resistance
    Article Snippet: A. tumefaciens GV3101 strains carrying the target plasmid are transiently expressed in the N. benthamiana and observed 48 h after A. tumefaciens GV3101 infiltration, detected with a confocal laser‐scanning microscopy (Nikon; A1HD25).



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    Kastle Therapeutics welzel j confocal laser scanning microscopy
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